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Home > Product > ELISA Kit > Human Interleukin 1Β, IL-1B ELISA Kit

Human Interleukin 1Β, IL-1B ELISA Kit

Human Interleukin 1Β, IL-1B ELISA Kit

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Product Summary

Size: 96T,48T

Sensitivity: 10.07pg/mL

Detection range: 20-6000pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

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Product Summary

Size: 96T,48T

Sensitivity: 10.07pg/mL

Detection range: 20-6000pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin 1 beta

Code

E0143Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

10.07pg/mL

Detection range

20-6000pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed: 10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed: 12794819).

UniProt accession

MASS(Da)

30,748

GeneID

3553

Synonyms

Catabolin;IL 1B;IL 1beta;IL-1 beta;IL1B;Interleukin-1 beta

Gene names

IL1B

Research area

Immunology; Microbiology; Cardiovascular; Metabolism

Target protein

IL1B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0143Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000pg/mL 2.364 2.272 2.23
2.18
2000pg/mL 1.378 1.317 1.275
1.256
1000pg/mL 0.807 0.724 0.682
0.642
500pg/mL 0.443 0.463 0.421
0.483
250pg/mL 0.195 0.208 0.166
0.222
0pg/mL 0.027 0.042 0
0.058

Precision

We measured random samples of E0143Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1899.9 61.65 3.2
Intra-Assay 2 18 821.3 40.18 4.9
Intra-Assay 3 18 1765.1 88.88 5

Protocols

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  • Size

    Code

    Price

  • 96T

    E0143Hu

    $458.00

  • 48T

    E0143Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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