Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Mouse Autophagy Related Protein 4B, ATG4B ELISA Kit

Mouse Autophagy Related Protein 4B, ATG4B ELISA Kit

Mouse Autophagy Related Protein 4B, ATG4B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Autophagy Related Protein 4B

Code

E2636Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity).

UniProt accession

MASS(Da)

44,375

GeneID

66615

Synonyms

ATG 4B;ATG4B;AUT-like 1 cysteine endopeptidase;Autophagin-1;Autophagy-related cysteine endopeptidase 1;Autophagy-related protein 4 homolog B;Cysteine protease ATG4B

Gene names

ATG4B

Research area

Cancer

Target protein

ATG4B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2636Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.16 2.19 2.166
2.221
6ng/ml 1.233 1.31 1.287
1.387
3ng/ml 0.567 0.586 0.563
0.605
1.5ng/ml 0.382 0.372 0.349
0.362
0.75ng/ml 0.151 0.134 0.11
0.117
0ng/ml 0.017 0.024 0
0.031

Precision

We measured random samples of E2636Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.4 0.2 2.7
Intra-Assay 2 18 4.4 0.19 4.2
Intra-Assay 3 18 2.3 0.1 4.3

Protocols

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 48T

    E2636Mo

    $320.00

  • 96T

    E2636Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up