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Home > Product > ELISA Kit > Mouse Poly Adp Ribose Polymerase 2, PARP2 ELISA Kit

Mouse Poly Adp Ribose Polymerase 2, PARP2 ELISA Kit

Mouse Poly Adp Ribose Polymerase 2, PARP2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 12.28ng/L

Detection range: 20-7000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 12.28ng/L

Detection range: 20-7000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Poly Adp Ribose Polymerase 2

Code

E2651Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

12.28ng/L

Detection range

20-7000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed: 10364231). Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (By similarity). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (By similarity). Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1 (By similarity). Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site (By similarity). PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks (By similarity). In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex (By similarity).

UniProt accession

MASS(Da)

63,397

GeneID

11546

Synonyms

ADP-ribosyltransferase diphtheria toxin-like 2;ADPRT-2;ARTD2;DNA ADP-ribosyltransferase PARP2;mPARP-2;NAD(+ ADP-ribosyltransferase 2;pADPRT-2;PARP 2;PARP2;PARP-2;Poly [ADP-ribose] polymerase 2;Poly[ADP-ribose] Synthase 2;Protein poly-ADP-ribosyltransferase PARP2;

Gene names

PARP2

Research area

Others

Target protein

PARP2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2651Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000ng/L 1.895 1.926 1.88
1.957
2000ng/L 1.029 0.981 0.935
0.934
1000ng/L 0.473 0.456 0.41
0.44
500ng/L 0.291 0.226 0.18
0.162
250ng/L 0.126 0.09 0.044
0.055
0ng/L 0.048 0.046 0
0.045

Precision

We measured random samples of E2651Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3393.1 63.72 1.9
Intra-Assay 2 18 1266.5 47.75 3.8
Intra-Assay 3 18 1887.5 78.33 4.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E2651Mo

    $320.00

  • 96T

    E2651Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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