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Home > Product > ELISA Kit > Mouse Fatty Acid Synthase, FASN ELISA Kit

Mouse Fatty Acid Synthase, FASN ELISA Kit

Mouse Fatty Acid Synthase, FASN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.57ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.57ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Fatty Acid Synthase

Code

E4250Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.57ng/L

Detection range

1-400ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.

UniProt accession

MASS(Da)

272,428

GeneID

14104

Synonyms

[Acyl-carrier-protein] S-malonyltransferase;3-hydroxyacyl-[acyl-carrier-protein] dehydratase;3-oxoacyl-[acyl-carrier-protein] reductase;3-oxoacyl-[acyl-carrier-protein] Synthase;Acyl-[acyl-carrier-protein] hydrolase;Enoyl-[acyl-carrier-protein] reductase;FASN;Fatty acid Synthase;Type I Fatty Acid Synthase [Includes: [Acyl-carrier-protein] S-acetyltransferase;

Gene names

FASN

Research area

Metabolism

Target protein

FASN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4250Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/L 2.347 2.232 2.186
2.117
120ng/L 1.703 1.704 1.658
1.705
60ng/L 1.259 1.209 1.163
1.158
30ng/L 0.637 0.645 0.599
0.652
15ng/L 0.414 0.391 0.345
0.368
0ng/L 0.056 0.046 0
0.036

Precision

We measured random samples of E4250Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 44.2 2.31 5.2
Intra-Assay 2 18 73.9 3.65 4.9
Intra-Assay 3 18 15 0.65 4.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E4250Mo

    $320.00

  • 96T

    E4250Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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