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Home > Product > ELISA Kit > Porcine Endothelin 1, ET-1 ELISA Kit

Porcine Endothelin 1, ET-1 ELISA Kit

Porcine Endothelin 1, ET-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.52ng/ml

Detection range: 3-700ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 1.52ng/ml

Detection range: 3-700ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Endothelin 1

Code

E0230Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.52ng/ml

Detection range

3-700ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Endothelins are endothelium-derived vasoconstrictor Peptides (PubMed: 2451132). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (By similarity).

UniProt accession

MASS(Da)

23,259

GeneID

396915

Synonyms

Big endothelin-1;EDN 1;EDN1;Endothelin-1;ET-1;PPET1;Preproendothelin-1

Gene names

EDN1

Research area

Cancer

Target protein

EDN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0230Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400ng/ml 1.959 1.888 1.827
1.817
200ng/ml 0.89 0.916 0.855
0.943
100ng/ml 0.443 0.537 0.476
0.63
50ng/ml 0.167 0.188 0.128
0.21
25ng/ml 0.146 0.132 0.071
0.117
0ng/ml 0.061 0.061 0
0.06

Precision

We measured random samples of E0230Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 103.3 4.54 4.4
Intra-Assay 2 18 216.5 9.85 4.5
Intra-Assay 3 18 340.7 8.26 2.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E0230Po

    $320.00

  • 96T

    E0230Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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