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Home > Product > ELISA Kit > Porcine Plasminogen Activator Inhibitor 1, PAI-1 ELISA Kit

Porcine Plasminogen Activator Inhibitor 1, PAI-1 ELISA Kit

Porcine Plasminogen Activator Inhibitor 1, PAI-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.053ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 0.053ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Plasminogen Activator Inhibitor 1

Code

E0264Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.053ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence (By similarity). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).

UniProt accession

MASS(Da)

45,450

GeneID

396945

Synonyms

Endothelial plasminogen activator inhibitor;PAI;PAI-1;Plasminogen activator inhibitor 1;Serpin E1;SERPINE 1;SERPINE1

Gene names

SERPINE1

Research area

Cell Biology

Target protein

SERPINE1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0264Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.213 2.269 2.205
2.325
12ng/mL 1.312 1.337 1.273
1.362
6ng/mL 0.869 0.896 0.831
0.923
3ng/mL 0.398 0.399 0.335
0.401
1.5ng/mL 0.251 0.234 0.17
0.218
0ng/mL 0.068 0.064 0
0.061

Precision

We measured random samples of E0264Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 15 0.56 3.7
Intra-Assay 2 18 3.9 0.23 6
Intra-Assay 3 18 7.9 0.51 6.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E0264Po

    $320.00

  • 96T

    E0264Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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