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Home > Product > ELISA Kit > Porcine Ghrelin, GHRL ELISA Kit

Porcine Ghrelin, GHRL ELISA Kit

Porcine Ghrelin, GHRL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.52ng/L

Detection range: 3-700ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 1.52ng/L

Detection range: 3-700ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Ghrelin

Code

E0335Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.52ng/L

Detection range

3-700ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation (By similarity). Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).

UniProt accession

MASS(Da)

12,786

GeneID

396728

Synonyms

Appetite-regulating hormone;Ghrelin;GHRL;Growth hormone secretagogue;Growth hormone-releasing Peptide;Motilin-related Peptide;Obestatin

Gene names

GHRL

Research area

Cardiovascular

Target protein

GHRL

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0335Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400ng/L 1.93 2.029 1.991
2.128
200ng/L 0.997 1.043 1.005
1.088
100ng/L 0.446 0.444 0.406
0.442
50ng/L 0.295 0.293 0.255
0.29
25ng/L 0.12 0.138 0.1
0.155
0ng/L 0.035 0.038 0
0.04

Precision

We measured random samples of E0335Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 139.4 7.04 5
Intra-Assay 2 18 223.3 8.88 4
Intra-Assay 3 18 319.2 9.41 2.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E0335Po

    $320.00

  • 96T

    E0335Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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