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Home > Product > ELISA Kit > Porcine Aspartate Aminotransferase, Cytoplasmic, AST ELISA Kit

Porcine Aspartate Aminotransferase, Cytoplasmic, AST ELISA Kit

Porcine Aspartate Aminotransferase, Cytoplasmic, AST ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.053ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 0.053ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Aspartate Aminotransferase, Cytoplasmic

Code

E0529Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.053ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain (By similarity).

UniProt accession

MASS(Da)

46475

GeneID

396967

Synonyms

Aspartate aminotransferase, cytoplasmic;cAspAT;cCAT;Cysteine aminotransferase, cytoplasmic;Cysteine transaminase, cytoplasmic;Glutamate oxaloacetate transaminase 1;GOT 1;GOT1;Transaminase A;

Gene names

GOT1

Research area

Signal Transduction

Target protein

GOT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0529Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.011 1.846 1.801
1.682
12ng/mL 1.084 0.976 0.93
0.867
6ng/mL 0.484 0.547 0.501
0.61
3ng/mL 0.298 0.276 0.23
0.254
1.5ng/mL 0.127 0.122 0.076
0.116
0ng/mL 0.059 0.046 0
0.033

Precision

We measured random samples of E0529Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 15.3 0.63 4.1
Intra-Assay 2 18 15.7 0.49 3.1
Intra-Assay 3 18 6.6 0.35 5.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E0529Po

    $320.00

  • 96T

    E0529Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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