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Home > Product > ELISA Kit > Porcine Caspase-3, CASP3 ELISA Kit

Porcine Caspase-3, CASP3 ELISA Kit

Porcine Caspase-3, CASP3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.148ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product Summary

Size: 48T,96T

Sensitivity: 0.148ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Porcine

Product overview

Full product name

Caspase-3

Code

E0542Po

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.148ng/mL

Detection range

0.3-90ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Porcine

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress.

UniProt accession

MASS(Da)

31,379

GeneID

397244

Synonyms

CASP 3;CASP3;CASP-3;Caspase-3;Caspase-3 subunit p12;Caspase-3 subunit p17

Gene names

CASP3

Research area

Others

Target protein

CASP3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0542Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/mL 2.11 2.078 2.039
2.046
24ng/mL 1.127 1.046 1.007
0.964
12ng/mL 0.488 0.489 0.451
0.491
6ng/mL 0.304 0.239 0.2
0.173
3ng/mL 0.108 0.122 0.083
0.136
0ng/mL 0.048 0.039 0
0.029

Precision

We measured random samples of E0542Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 9.1 0.37 4.1
Intra-Assay 2 18 27.6 1.31 4.8
Intra-Assay 3 18 29.2 0.81 2.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E0542Po

    $320.00

  • 96T

    E0542Po

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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