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Home > Product > ELISA Kit > Bovine Deoxyribonuclease 1, DNASE-1 ELISA Kit

Bovine Deoxyribonuclease 1, DNASE-1 ELISA Kit

Bovine Deoxyribonuclease 1, DNASE-1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 3.91ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 3.91ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Deoxyribonuclease 1

Code

E0173Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

3.91ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed: 4976790, PubMed: 5166750, PubMed: 3352748, PubMed: 2395459). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed: 2395459). Binds specifically to G-actin and blocks actin polymerization (PubMed: 2395459). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).

UniProt accession

MASS(Da)

31,346

GeneID

282217

Synonyms

Deoxyribonuclease I;Deoxyribonuclease-1;DNASE 1;DNase I;DNASE1

Gene names

DNASE1

Research area

Epigenetics and Nuclear Signaling

Target protein

DNASE1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0173Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.391 2.304 2.25
2.217
400ng/L 1.249 1.211 1.157
1.173
200ng/L 0.722 0.687 0.633
0.652
100ng/L 0.5 0.473 0.419
0.446
50ng/L 0.203 0.214 0.16
0.225
0ng/L 0.045 0.054 0
0.063

Precision

We measured random samples of E0173Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 447.8 9.83 2.2
Intra-Assay 2 18 107.2 7.37 6.9
Intra-Assay 3 18 307 12.77 4.2

Protocols

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  • Size

    Code

    Price

  • 96T

    E0173Bo

    $458.00

  • 48T

    E0173Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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