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Home > Product > ELISA Kit > Human Lymphotoxin-alpha, LTA ELISA Kit

Human Lymphotoxin-alpha, LTA ELISA Kit

Human Lymphotoxin-alpha, LTA ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.16pmol/L

Detection range: 0.3-90pmol/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.16pmol/L

Detection range: 0.3-90pmol/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Lymphotoxin-Alpha

Code

E0170Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.16pmol/L

Detection range

0.3-90pmol/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (PubMed: 9462508). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.

UniProt accession

MASS(Da)

22,297

GeneID

4049

Synonyms

LT-alpha;Lymphotoxin-alpha;TNF B;TNF beta;TNFB;TNF-beta;Tumor necrosis factor ligand superfamily member 1

Gene names

TNFB

Research area

Immunology; Signal Transduction; Cancer

Target protein

TNFB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0170Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48pmol/L 2.664 2.642 2.604
2.62
24pmol/L 1.481 1.465 1.427
1.448
12pmol/L 0.93 0.885 0.847
0.839
6pmol/L 0.485 0.449 0.412
0.414
3pmol/L 0.212 0.232 0.194
0.253
0pmol/L 0.039 0.038 0
0.037

Precision

We measured random samples of E0170Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 30.5 1.16 3.8
Intra-Assay 2 18 18.5 1.1 5.9
Intra-Assay 3 18 4.5 0.21 4.7

Protocols

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  • Size

    Code

    Price

  • 96T

    E0170Hu

    $458.00

  • 48T

    E0170Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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