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Home > Product > ELISA Kit > Rabbit Insulin-like Growth Factors 1, IGF-1 ELISA Kit

Rabbit Insulin-like Growth Factors 1, IGF-1 ELISA Kit

Rabbit Insulin-like Growth Factors 1, IGF-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.25ng/ml

Detection range: 5-1000ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product Summary

Size: 48T,96T

Sensitivity: 2.25ng/ml

Detection range: 5-1000ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product overview

Full product name

Insulin-like Growth Factors 1

Code

E0144Rb

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.25ng/ml

Detection range

5-1000ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rabbit

Storage

2-8ºC

Assay time

1h 30m

Background

The Insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (By similarity).

UniProt accession

MASS(Da)

16,092

GeneID

100008668

Synonyms

IGF 1;IGF1;IGF-I;Insulin-like growth factor I;Somatomedin

Gene names

IGF1

Research area

Signal Transduction

Target protein

IGF1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0144Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/ml 2.178 2.238 2.189
2.298
320ng/ml 1.432 1.471 1.423
1.511
160ng/ml 0.797 0.788 0.74
0.78
80ng/ml 0.449 0.474 0.426
0.5
40ng/ml 0.283 0.263 0.214
0.243
0ng/ml 0.041 0.049 0
0.056

Precision

We measured random samples of E0144Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 109.1 7.38 6.8
Intra-Assay 2 18 239.8 16.19 6.8
Intra-Assay 3 18 412.7 15.2 3.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E0144Rb

    $320.00

  • 96T

    E0144Rb

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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