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Home > Product > ELISA Kit > Human Vascular Cell Adhesion Molecule 1, VCAM-1 ELISA Kit

Human Vascular Cell Adhesion Molecule 1, VCAM-1 ELISA Kit

Human Vascular Cell Adhesion Molecule 1, VCAM-1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.23ng/ml

Detection range: 0.5-200ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.23ng/ml

Detection range: 0.5-200ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Vascular Cell Adhesion Molecule 1

Code

E0203Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.23ng/ml

Detection range

0.5-200ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

UniProt accession

MASS(Da)

81,276

GeneID

7412

Synonyms

CD antigen CD106;INCAM-100;Vascular cell adhesion protein 1;VCAM 1;V-CAM 1;VCAM1;VCAM-1

Gene names

VCAM1

Research area

Cancer

Target protein

VCAM1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0203Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/ml 2.239 2.13 2.081
2.021
60ng/ml 1.395 1.338 1.29
1.282
30ng/ml 0.902 0.874 0.825
0.845
15ng/ml 0.444 0.483 0.434
0.521
7.5ng/ml 0.309 0.282 0.234
0.255
0ng/ml 0.037 0.049 0
0.061

Precision

We measured random samples of E0203Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 14.3 1.03 7.2
Intra-Assay 2 18 39.5 2.75 7
Intra-Assay 3 18 68.2 3.32 4.9

Protocols

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  • Size

    Code

    Price

  • 96T

    E0203Hu

    $458.00

  • 48T

    E0203Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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