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Home > Product > ELISA Kit > Rabbit Bone Morphogenetic Protein 2, BMP2 ELISA Kit

Rabbit Bone Morphogenetic Protein 2, BMP2 ELISA Kit

Rabbit Bone Morphogenetic Protein 2, BMP2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/L

Detection range: 0.5-200ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/L

Detection range: 0.5-200ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product overview

Full product name

Bone Morphogenetic Protein 2

Code

E0318Rb

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.26ng/L

Detection range

0.5-200ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rabbit

Storage

2-8ºC

Assay time

1h 30m

Background

Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.

UniProt accession

MASS(Da)

44,664

GeneID

100009349

Synonyms

BMP 2;BMP2;BMP-2;Bone morphogenetic protein 2

Gene names

BMP2

Research area

Cardiovascular; Signal Transduction

Target protein

BMP2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0318Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/L 2.325 2.178 2.124
2.03
60ng/L 1.692 1.766 1.712
1.839
30ng/L 1.166 1.144 1.091
1.123
15ng/L 0.746 0.736 0.683
0.727
7.5ng/L 0.359 0.398 0.345
0.438
0ng/L 0.047 0.053 0
0.06

Precision

We measured random samples of E0318Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 33.7 1.37 4.1
Intra-Assay 2 18 9.1 0.51 5.5
Intra-Assay 3 18 16 0.92 5.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E0318Rb

    $320.00

  • 96T

    E0318Rb

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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