Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0103Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 200ng/L | 2.265 | 2.174 | 2.127 |
| 2.083 | |||
| 100ng/L | 1.545 | 1.65 | 1.603 |
| 1.754 | |||
| 50ng/L | 1.14 | 1.071 | 1.024 |
| 1.002 | |||
| 25ng/L | 0.675 | 0.719 | 0.672 |
| 0.764 | |||
| 12.5ng/L | 0.441 | 0.431 | 0.384 |
| 0.422 | |||
| 0ng/L | 0.047 | 0.047 | 0 |
| 0.048 |
Precision
We measured random samples of E0103Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 119.1 | 7.14 | 6 |
| Intra-Assay | 2 | 18 | 58.6 | 3.21 | 5.5 |
| Intra-Assay | 3 | 18 | 15.3 | 0.87 | 5.7 |
Protocols
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