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Home > Product > ELISA Kit > Rat Complement 4, C4 ELISA Kit

Rat Complement 4, C4 ELISA Kit

Rat Complement 4, C4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.42ng/mL

Detection range: 7-1500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 3.42ng/mL

Detection range: 7-1500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Complement 4

Code

E0160Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.42ng/mL

Detection range

7-1500ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes (By similarity). Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory processes. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

UniProt accession

MASS(Da)

192163

GeneID

24233

Synonyms

C 4;C4;C4a anaphylatoxin;Complement C4 alpha chain;Complement C4 Complement C4 beta chain;Complement C4 gamma chain

Gene names

C4

Research area

Others

Target protein

C4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0160Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/mL 2.239 2.308 2.239
2.376
400ng/mL 1.223 1.342 1.273
1.461
200ng/mL 0.871 0.832 0.763
0.792
100ng/mL 0.462 0.448 0.38
0.435
50ng/mL 0.314 0.279 0.21
0.244
0ng/mL 0.059 0.069 0
0.079

Precision

We measured random samples of E0160Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 553.4 19.51 3.5
Intra-Assay 2 18 202.3 9.37 4.6
Intra-Assay 3 18 176.7 12.5 7.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E0160Ra

    $320.00

  • 96T

    E0160Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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