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Home > Product > ELISA Kit > Rat Interferon-inducible Protein 10, IP-10 ELISA Kit

Rat Interferon-inducible Protein 10, IP-10 ELISA Kit

Rat Interferon-inducible Protein 10, IP-10 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.64pg/ml

Detection range: 5-1500pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 2.64pg/ml

Detection range: 5-1500pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Interferon-Inducible Protein 10

Code

E0243Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.64pg/ml

Detection range

5-1500pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (By similarity). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (By similarity). Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity) (PubMed: 30257241, PubMed: 30448292).

UniProt accession

MASS(Da)

10,729

GeneID

245920

Synonyms

10 kDa interferon gamma-induced protein;C-X-C motif chemokine 10;CXCL 10;CXCL10;Gamma-IP10;Interferon-inducible protein 10;IP-10;Protein Mob-1;Small-inducible cytokine B10;

Gene names

CXCL10

Research area

Immunology

Target protein

CXCL10

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0243Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800pg/ml 2.288 2.209 2.166
2.13
400pg/ml 1.217 1.299 1.255
1.38
200pg/ml 0.729 0.75 0.707
0.772
100pg/ml 0.384 0.378 0.335
0.372
50pg/ml 0.178 0.183 0.14
0.188
0pg/ml 0.033 0.043 0
0.054

Precision

We measured random samples of E0243Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 475.5 10.6 2.2
Intra-Assay 2 18 317.7 17.4 5.5
Intra-Assay 3 18 182.8 9.78 5.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E0243Ra

    $320.00

  • 96T

    E0243Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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