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Home > Product > ELISA Kit > Rat Osteonectin, ON ELISA Kit

Rat Osteonectin, ON ELISA Kit

Rat Osteonectin, ON ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.54ng/mL

Detection range: 1-400ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.54ng/mL

Detection range: 1-400ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Osteonectin

Code

E0278Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.54ng/mL

Detection range

1-400ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

UniProt accession

MASS(Da)

34,963

GeneID

25353

Synonyms

Bone sialoprotein 1;Osteopontin;Secreted phosphoprotein 1;SPP 1;SPP1;SPP-1

Gene names

SPP1

Research area

Metabolism

Target protein

SPP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0278Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/mL 1.848 1.867 1.826
1.885
120ng/mL 1.016 1.043 1.003
1.071
60ng/mL 0.537 0.561 0.521
0.585
30ng/mL 0.164 0.19 0.15
0.217
15ng/mL 0.168 0.152 0.112
0.137
0ng/mL 0.029 0.04 0
0.051

Precision

We measured random samples of E0278Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 47.3 1.73 3.7
Intra-Assay 2 18 88.8 3.72 4.2
Intra-Assay 3 18 197.5 5.78 2.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E0278Ra

    $320.00

  • 96T

    E0278Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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