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Home > Product > ELISA Kit > Rat Tissue Inhibitors of Metalloproteinase 1, TIMP-1 ELISA Kit

Rat Tissue Inhibitors of Metalloproteinase 1, TIMP-1 ELISA Kit

Rat Tissue Inhibitors of Metalloproteinase 1, TIMP-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.02ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.02ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Tissue Inhibitors of Metalloproteinase 1

Code

E0322Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.02ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Also stimulates steroidogenesis by Leydig and ovarian granuloma cells; procathepsin L is required for maximal activity.

UniProt accession

MASS(Da)

23,794

GeneID

116510

Synonyms

Metalloproteinase inhibitor 1;TIMP 1;TIMP1;TIMP-1;Tissue inhibitor of metalloproteinases 1

Gene names

TIMP1

Research area

Cardiovascular

Target protein

TIMP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0322Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 1.965 2.034 1.986
2.103
4ng/ml 0.951 1.016 0.968
1.082
2ng/ml 0.612 0.501 0.453
0.391
1ng/ml 0.219 0.246 0.198
0.274
0.5ng/ml 0.135 0.146 0.098
0.157
0ng/ml 0.059 0.048 0
0.037

Precision

We measured random samples of E0322Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.5 0.17 2.7
Intra-Assay 2 18 1.9 0.11 5.6
Intra-Assay 3 18 4.4 0.22 5

Protocols

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  • Size

    Code

    Price

  • 48T

    E0322Ra

    $320.00

  • 96T

    E0322Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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