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Home > Product > ELISA Kit > Rat Haptoglobin, HPT ELISA Kit

Rat Haptoglobin, HPT ELISA Kit

Rat Haptoglobin, HPT ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022μg/mL

Detection range: 0.05-20ug/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.022μg/mL

Detection range: 0.05-20ug/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Haptoglobin

Code

E0357Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022μg/mL

Detection range

0.05-20ug/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).

UniProt accession

MASS(Da)

38,563

GeneID

24464

Synonyms

Haptoglobin;Haptoglobin alpha chain;Haptoglobin beta chain;HP;Liver regeneration-related protein LRRG173

Gene names

HP

Research area

Cardiovascular

Target protein

HP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0357Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12μg/mL 2.124 2.189 2.143
2.255
6μg/mL 1.375 1.265 1.218
1.154
3μg/mL 0.816 0.837 0.791
0.859
1.5μg/mL 0.479 0.481 0.435
0.482
0.75μg/mL 0.255 0.231 0.185
0.208
0μg/mL 0.054 0.046 0
0.039

Precision

We measured random samples of E0357Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.4 0.1 7.1
Intra-Assay 2 18 8.2 0.24 3
Intra-Assay 3 18 3.6 0.24 6.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E0357Ra

    $320.00

  • 96T

    E0357Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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