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Home > Product > ELISA Kit > Rat Granzymes B, GZMS-B ELISA Kit

Rat Granzymes B, GZMS-B ELISA Kit

Rat Granzymes B, GZMS-B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.29ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 3.29ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Granzymes B

Code

E0403Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.29ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

UniProt accession

MASS(Da)

27,326

GeneID

1.0036146817153E+14

Synonyms

Fragmentin;Granzyme B;GZMB;Natural killer cell protease 1;RNKP-1

Gene names

GZMB

Research area

Others

Target protein

GZMB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0403Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected

Precision

We measured random samples of E0403Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 492.26 16.26 3.3
Intra-Assay 2 18 90.19 5.74 6.36
Intra-Assay 3 18 336.67 17.15 5.09

Protocols

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  • Size

    Code

    Price

  • 48T

    E0403Ra

    $320.00

  • 96T

    E0403Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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