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Home > Product > ELISA Kit > Rat Tryptase, TPS ELISA Kit

Rat Tryptase, TPS ELISA Kit

Rat Tryptase, TPS ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Tryptase

Code

E0427Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity (By similarity).

UniProt accession

MASS(Da)

30,400

GeneID

54271

Synonyms

Mast cell protease 7;rMCP-7;TPSAB 1;TPSAB1;Tryptase;Tryptase alpha/beta-1;Tryptase, skin

Gene names

TPSAB1

Research area

Immunology

Target protein

TPSAB1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0427Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/ml 2.108 2.085 2.029
2.062
3.2ng/ml 1.713 1.718 1.662
1.724
1.6ng/ml 1.166 1.134 1.078
1.102
0.8ng/ml 0.673 0.678 0.622
0.683
0.4ng/ml 0.43 0.434 0.378
0.439
0ng/ml 0.065 0.056 0
0.048

Precision

We measured random samples of E0427Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.7 0.13 7.6
Intra-Assay 2 18 4.1 0.27 6.5
Intra-Assay 3 18 0.6 0.03 4.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E0427Ra

    $320.00

  • 96T

    E0427Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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