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Home > Product > ELISA Kit > Rat Coagulation Factor 12, F12 ELISA Kit

Rat Coagulation Factor 12, F12 ELISA Kit

Rat Coagulation Factor 12, F12 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.156ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.156ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Coagulation Factor 12

Code

E0492Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.156ng/mL

Detection range

0.3-90ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (By similarity).

UniProt accession

MASS(Da)

65844

GeneID

306761

Synonyms

Coagulation factor XII;Coagulation factor XIIa light chain;F12;HAF Coagulation factor XIIa heavy chain;Hageman factor

Gene names

F12

Research area

Others

Target protein

F12

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0492Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/mL 2.269 2.193 2.147
2.117
24ng/mL 1.4 1.324 1.279
1.248
12ng/mL 0.852 0.766 0.721
0.68
6ng/mL 0.44 0.471 0.426
0.503
3ng/mL 0.202 0.192 0.147
0.182
0ng/mL 0.039 0.046 0
0.052

Precision

We measured random samples of E0492Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.7 0.38 5.7
Intra-Assay 2 18 14.3 0.87 6.1
Intra-Assay 3 18 35.5 1.51 4.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E0492Ra

    $320.00

  • 96T

    E0492Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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