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Home > Product > ELISA Kit > Rat Tumor Necrosis Factor Β, TNF-B ELISA Kit

Rat Tumor Necrosis Factor Β, TNF-B ELISA Kit

Rat Tumor Necrosis Factor Β, TNF-B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.02pg/ml

Detection range: 2-600pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 1.02pg/ml

Detection range: 2-600pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Tumor Necrosis Factor beta

Code

E0765Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.02pg/ml

Detection range

2-600pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (By similarity). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.

UniProt accession

MASS(Da)

22,112

GeneID

25008

Synonyms

LT-alpha;Lymphotoxin-alpha;TNB;TNF B;TNF beta;TNFB;TNF-beta;Tumor necrosis factor ligand superfamily member 1

Gene names

TNFB

Research area

Immunology; Signal Transduction; Cancer

Target protein

TNFB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0765Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400pg/ml 2.273 2.207 2.159
2.142
200pg/ml 1.437 1.479 1.43
1.521
100pg/ml 0.673 0.608 0.559
0.543
50pg/ml 0.462 0.4 0.351
0.338
25pg/ml 0.116 0.161 0.112
0.206
0pg/ml 0.059 0.049 0
0.039

Precision

We measured random samples of E0765Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 259.9 9.37 3.6
Intra-Assay 2 18 152.4 6.54 4.3
Intra-Assay 3 18 83.8 3.83 4.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E0765Ra

    $320.00

  • 96T

    E0765Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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