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Home > Product > ELISA Kit > Rat Androgen Receptor, AR ELISA Kit

Rat Androgen Receptor, AR ELISA Kit

Rat Androgen Receptor, AR ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.029ng/ml

Detection range: 0.05-35ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.029ng/ml

Detection range: 0.05-35ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Androgen Receptor

Code

E0909Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.029ng/ml

Detection range

0.05-35ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation. Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3 (By similarity).

UniProt accession

MASS(Da)

98,218

GeneID

24208

Synonyms

Androgen receptor;AR;Dihydrotestosterone receptor;Nuclear receptor subfamily 3 group C member 4

Gene names

AR

Research area

Cancer

Target protein

AR

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0909Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 1.723 1.865 1.823
2.007
10ng/ml 0.961 0.985 0.944
1.009
5ng/ml 0.596 0.525 0.483
0.454
2.5ng/ml 0.274 0.254 0.212
0.234
1.25ng/ml 0.075 0.099 0.058
0.123
0ng/ml 0.052 0.042 0
0.031

Precision

We measured random samples of E0909Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 17.7 0.65 3.7
Intra-Assay 2 18 10 0.52 5.2
Intra-Assay 3 18 4.2 0.19 4.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E0909Ra

    $320.00

  • 96T

    E0909Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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