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Home > Product > ELISA Kit > Rat Sorbitol Dehydrogenase, SDH ELISA Kit

Rat Sorbitol Dehydrogenase, SDH ELISA Kit

Rat Sorbitol Dehydrogenase, SDH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.056mIU/mL

Detection range: 0.1-40mIU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.056mIU/mL

Detection range: 0.1-40mIU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Sorbitol Dehydrogenase

Code

E0914Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.056mIU/mL

Detection range

0.1-40mIU/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols (By similarity). Is active with D-sorbitol (D-glucitol) leading to the C2-oxidized product D-fructose (PubMed: 6862079). Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility (By similarity).

UniProt accession

MASS(Da)

38,235

GeneID

24788

Synonyms

L-iditol 2-dehydrogenase;Polyol dehydrogenase;SDH;Sorbitol dehydrogenase;SORD;XDH;Xylitol dehydrogenase

Gene names

SORD

Research area

Others

Target protein

SORD

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0914Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24mIU/mL 1.808 1.812 1.762
1.815
12mIU/mL 0.884 0.886 0.836
0.888
6mIU/mL 0.451 0.486 0.437
0.522
3mIU/mL 0.251 0.266 0.217
0.282
1.5mIU/mL 0.12 0.129 0.079
0.139
0mIU/mL 0.06 0.05 0
0.039

Precision

We measured random samples of E0914Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 14.6 0.61 4.1
Intra-Assay 2 18 20.7 0.57 2.8
Intra-Assay 3 18 7 0.34 4.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E0914Ra

    $320.00

  • 96T

    E0914Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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