Product overview
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Background
Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid Peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive Peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological Peptides, such as apelins, casomorphins and dynorphin A. Plays an important role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 in intestine, regulating trafficking, expression on the cell surface, and its catalytic activity.
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Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0968Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 64ng/ml | 1.965 | 1.91 | 1.85 |
| 1.854 | |||
| 32ng/ml | 0.918 | 0.972 | 0.913 |
| 1.027 | |||
| 16ng/ml | 0.45 | 0.496 | 0.436 |
| 0.543 | |||
| 8ng/ml | 0.239 | 0.246 | 0.186 |
| 0.252 | |||
| 4ng/ml | 0.135 | 0.144 | 0.084 |
| 0.153 | |||
| 0ng/ml | 0.055 | 0.06 | 0 |
| 0.064 |
Precision
We measured random samples of E0968Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 60.9 | 1.52 | 2.5 |
| Intra-Assay | 2 | 18 | 26.3 | 1.24 | 4.7 |
| Intra-Assay | 3 | 18 | 18.6 | 0.95 | 5.1 |
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