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Home > Product > ELISA Kit > Rat Apelin, AP ELISA Kit

Rat Apelin, AP ELISA Kit

Rat Apelin, AP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.524ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.524ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Apelin

Code

E1026Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.524ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Endogenous ligand for the apelin receptor (APLNR) (PubMed: 11336787, PubMed: 11359874, PubMed: 26611206). Drives internalization of the apelin receptor (PubMed: 11359874). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (PubMed: 11336787). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity). Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (PubMed: 26611206). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (PubMed: 10617103, PubMed: 11359874).

UniProt accession

MASS(Da)

8,677

GeneID

58812

Synonyms

Apelin;Apelin-13;Apelin-28;Apelin-31;Apelin-36;APJ endogenous ligand;APLN

Gene names

APLN

Research area

Others

Target protein

APLN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1026Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.15 2.198 2.153
2.246
12ng/mL 1.367 1.25 1.205
1.134
6ng/mL 0.803 0.736 0.69
0.669
3ng/mL 0.426 0.403 0.357
0.38
1.5ng/mL 0.191 0.203 0.157
0.215
0ng/mL 0.063 0.046 0
0.028

Precision

We measured random samples of E1026Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 15.5 0.58 3.7
Intra-Assay 2 18 3.6 0.23 6.4
Intra-Assay 3 18 6.7 0.37 5.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1026Ra

    $320.00

  • 96T

    E1026Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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