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Home > Product > ELISA Kit > Rat Mesencephalic Astrocyte-derived Neurotrophic Factor, MANF ELISA Kit

Rat Mesencephalic Astrocyte-derived Neurotrophic Factor, MANF ELISA Kit

Rat Mesencephalic Astrocyte-derived Neurotrophic Factor, MANF ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.057ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.057ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Mesencephalic Astrocyte-Derived Neurotrophic Factor

Code

E1068Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.057ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (By similarity). Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (PubMed: 16462600). Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (PubMed: 16462600). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (By similarity). Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (By similarity). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (By similarity). Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (By similarity). Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (By similarity).

UniProt accession

MASS(Da)

20,388

GeneID

315989

Synonyms

Arginine-rich protein;MANF;Mesencephalic astrocyte-derived neurotrophic factor;Protein ARMET

Gene names

MANF

Research area

Others

Target protein

MANF

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1068Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.144 2.185 2.133
2.227
12ng/mL 1.491 1.485 1.433
1.478
6ng/mL 0.618 0.665 0.612
0.711
3ng/mL 0.308 0.329 0.277
0.35
1.5ng/mL 0.154 0.144 0.092
0.134
0ng/mL 0.048 0.052 0
0.056

Precision

We measured random samples of E1068Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 12.2 0.41 3.3
Intra-Assay 2 18 7.5 0.35 4.7
Intra-Assay 3 18 4 0.14 3.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1068Ra

    $320.00

  • 96T

    E1068Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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