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Home > Product > ELISA Kit > Rat Glutamate Oxaloacetate Transaminase 1, GOT1 ELISA Kit

Rat Glutamate Oxaloacetate Transaminase 1, GOT1 ELISA Kit

Rat Glutamate Oxaloacetate Transaminase 1, GOT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.07U/L

Detection range: 2-600U/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 1.07U/L

Detection range: 2-600U/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Glutamate Oxaloacetate Transaminase 1

Code

E1141Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.07U/L

Detection range

2-600U/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

UniProt accession

MASS(Da)

46,429

GeneID

24401

Synonyms

Aspartate aminotransferase, cytoplasmic;cAspAT;cCAT;Cysteine aminotransferase, cytoplasmic;Cysteine transaminase, cytoplasmic;Glutamate oxaloacetate transaminase 1;GOT 1;GOT1;Transaminase A;

Gene names

GOT1

Research area

Signal Transduction

Target protein

GOT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1141Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400U/L 2.161 2.116 2.069
2.071
200U/L 1.224 1.314 1.268
1.404
100U/L 0.832 0.834 0.788
0.837
50U/L 0.378 0.432 0.385
0.486
25U/L 0.226 0.202 0.155
0.177
0U/L 0.032 0.046 0
0.061

Precision

We measured random samples of E1141Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 166.8 9.2 5.5
Intra-Assay 2 18 208.4 7.41 3.6
Intra-Assay 3 18 48 2.63 5.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1141Ra

    $320.00

  • 96T

    E1141Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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