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Home > Product > ELISA Kit > Rat Glutamate[NMDA] Receptor Subunit Epsilon-2, GRIN2B ELISA Kit

Rat Glutamate[NMDA] Receptor Subunit Epsilon-2, GRIN2B ELISA Kit

Rat Glutamate[NMDA] Receptor Subunit Epsilon-2, GRIN2B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.01ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.01ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Glutamate[Nmda]Receptor Subunit Epsilon-2

Code

E1204Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.01ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion Channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate Channel inhibition by Mg(2+) (PubMed: 1350383, PubMed: 19910922, PubMed: 21677647, PubMed: 24876489, PubMed: 27135925, PubMed: 27916457). Sensitivity to glutamate and Channel kinetics depend on the subunit composition (Probable). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor Channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Contributes to neural pattern formation in the developing brain. Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity (By similarity).

UniProt accession

MASS(Da)

166,071

GeneID

24410

Synonyms

GluN2B;Glutamate [NMDA] receptor subunit epsilon-2;Glutamate receptor ionotropic, NMDA 2B;GRIN 2B;GRIN2B;NMDAR2B;N-methyl D-aspartate receptor subtype 2B;NR2B

Gene names

GRIN2B

Research area

Others

Target protein

GRIN2B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1204Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/ml 2.262 2.186 2.158
2.11
3.2ng/ml 1.392 1.358 1.331
1.324
1.6ng/ml 0.573 0.572 0.544
0.571
0.8ng/ml 0.437 0.371 0.343
0.304
0.4ng/ml 0.175 0.155 0.128
0.136
0ng/ml 0.032 0.028 0
0.024

Precision

We measured random samples of E1204Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.2 0.08 3.7
Intra-Assay 2 18 3.5 0.11 3.1
Intra-Assay 3 18 0.9 0.05 5.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E1204Ra

    $320.00

  • 96T

    E1204Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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