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Home > Product > ELISA Kit > Rat Progranulin, PGRN ELISA Kit

Rat Progranulin, PGRN ELISA Kit

Rat Progranulin, PGRN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/ml

Detection range: 0.5-200ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/ml

Detection range: 0.5-200ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Progranulin

Code

E1222Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.26ng/ml

Detection range

0.5-200ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (By similarity). Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes (By similarity). Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (By similarity). In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (By similarity). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (By similarity). Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (By similarity). [Granulin-3]: Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8. [Granulin-7]: Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.

UniProt accession

MASS(Da)

63,370

GeneID

29143

Synonyms

Acrogranin;Epithelin precursor;Epithelin-1;Epithelin-2;Granulin A;Granulin B;Granulin C;Granulin D;Granulin E;Granulin F;Granulin G;Granulin precursor;Granulin-1;Granulin-2;Granulin-3;Granulin-4;Granulin-5;Granulin-6;Granulin-7;GRN;Paragranulin;PEPI;PGRN;Proepithelin;Progranulin

Gene names

GRN

Research area

Neuroscience; Immunology

Target protein

GRN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1222Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/ml 2.201 2.114 2.053
2.028
60ng/ml 1.288 1.403 1.341
1.518
30ng/ml 0.781 0.821 0.76
0.861
15ng/ml 0.542 0.535 0.473
0.527
7.5ng/ml 0.291 0.293 0.231
0.294
0ng/ml 0.052 0.061 0
0.071

Precision

We measured random samples of E1222Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 94.4 4.2 4.5
Intra-Assay 2 18 33.2 2.25 6.8
Intra-Assay 3 18 12.7 0.76 6

Protocols

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  • Size

    Code

    Price

  • 48T

    E1222Ra

    $320.00

  • 96T

    E1222Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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