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Home > Product > ELISA Kit > Rat Neprilysin, NEP ELISA Kit

Rat Neprilysin, NEP ELISA Kit

Rat Neprilysin, NEP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.41ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 9.41ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Neprilysin

Code

E1226Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.41ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed: 2966343) (By similarity). Biologically important in the destruction of opioid Peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed: 2966343) (By similarity). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed: 2966343) (By similarity). Involved in the degradation of the atrial natriuretic factor (ANF) (PubMed: 2966343). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (By similarity).

UniProt accession

MASS(Da)

85,795

GeneID

24590

Synonyms

Atriopeptidase;CD antigen CD10;Enkephalinase;MME;NEP;Neprilysin;Neutral endopeptidase;Neutral endopeptidase 24.11;SFE;Skin fibroblast elastase

Gene names

MME

Research area

Others

Target protein

MME

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1226Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.262 2.263 2.213
2.264
1600ng/L 1.519 1.617 1.567
1.716
800ng/L 0.969 0.993 0.942
1.017
400ng/L 0.737 0.694 0.644
0.651
200ng/L 0.469 0.481 0.43
0.493
0ng/L 0.042 0.05 0
0.059

Precision

We measured random samples of E1226Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1437.4 55.41 3.9
Intra-Assay 2 18 707.1 53.16 7.5
Intra-Assay 3 18 440.2 30.15 6.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E1226Ra

    $320.00

  • 96T

    E1226Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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