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Home > Product > ELISA Kit > Rat Mothers Against Decapentaplegic Homolog 3, SMAD3 ELISA Kit

Rat Mothers Against Decapentaplegic Homolog 3, SMAD3 ELISA Kit

Rat Mothers Against Decapentaplegic Homolog 3, SMAD3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.31ng/L

Detection range: 21.01-7500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 10.31ng/L

Detection range: 21.01-7500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Mothers Against Decapentaplegic Homolog 3

Code

E1259Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.31ng/L

Detection range

21.01-7500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).

UniProt accession

MASS(Da)

48,081

GeneID

25631

Synonyms

MAD homolog 3;Mad3;Mothers against decapentaplegic homolog 3;Mothers against DPP homolog 3;SMAD 3;SMAD family member 3;SMAD3

Gene names

SMAD3

Research area

Signal Transduction; Epigenetics and Nuclear Signaling; Stem Cells; Cancer

Target protein

SMAD3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1259Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000ng/L 2.297 2.199 2.162
2.101
2000ng/L 1.49 1.533 1.496
1.577
1000ng/L 0.633 0.698 0.661
0.763
500ng/L 0.381 0.384 0.347
0.388
250ng/L 0.134 0.161 0.124
0.189
0ng/L 0.049 0.037 0
0.025

Precision

We measured random samples of E1259Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1419 46.24 3.3
Intra-Assay 2 18 1806.9 54.13 3
Intra-Assay 3 18 705.7 28.47 4

Protocols

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  • Size

    Code

    Price

  • 48T

    E1259Ra

    $320.00

  • 96T

    E1259Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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