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Home > Product > ELISA Kit > Rat Sterol Regulatory Element-binding Protein 1, SREBP1 ELISA Kit

Rat Sterol Regulatory Element-binding Protein 1, SREBP1 ELISA Kit

Rat Sterol Regulatory Element-binding Protein 1, SREBP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.16ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.16ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Sterol Regulatory Element-Binding Protein 1

Code

E1312Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.16ng/ml

Detection range

0.3-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

[Sterol regulatory element-binding protein 1]: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane (By similarity). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis and lipid homeostasis (By similarity). [Processed sterol regulatory element-binding protein 1]: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis and lipid homeostasis (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') (PubMed: 7739539). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') (By similarity). Regulates the promoters of genes involved in cholesterol biosynthesis and the LDL receptor (LDLR) pathway of sterol regulation (By similarity). [Isoform SREBP-1A]: Isoform expressed only in select tissues, which has higher transcriptional activity compared to SREBP-1C (By similarity). Able to stimulate both lipogenic and cholesterogenic gene expression (By similarity). Has a role in the nutritional regulation of fatty acids and triglycerides in lipogenic organs such as the liver. Required for innate immune response in macrophages by regulating lipid metabolism (By similarity). [Isoform SREBP-1C]: Predominant isoform expressed in most tissues, which has weaker transcriptional activity compared to isoform SREBP-1A (By similarity). Primarily controls expression of lipogenic gene (By similarity). Strongly activates global lipid synthesis in rapidly growing cells (By similarity).

UniProt accession

MASS(Da)

120,521

GeneID

78968

Synonyms

ADD1;Adipocyte determination- and differentiation-dependent factor 1;Processed sterol regulatory element-binding protein 1;SREBF 1;SREBF1;SREBP-1;Sterol regulatory element-binding protein 1;Sterol regulatory element-binding transcription factor 1;Transcription factor SREBF1;

Gene names

SREBF1

Research area

Metabolism

Target protein

SREBF1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1312Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 2.163 2.18 2.14
2.197
24ng/ml 1.234 1.236 1.197
1.238
12ng/ml 0.574 0.677 0.638
0.78
6ng/ml 0.362 0.379 0.34
0.396
3ng/ml 0.152 0.164 0.125
0.176
0ng/ml 0.043 0.039 0
0.036

Precision

We measured random samples of E1312Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 29 1.06 3.7
Intra-Assay 2 18 14.9 0.63 4.3
Intra-Assay 3 18 9.3 0.57 6.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E1312Ra

    $320.00

  • 96T

    E1312Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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