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Home > Product > ELISA Kit > Rat Calpain-2 Catalytic Subunit, CAPN2 ELISA Kit

Rat Calpain-2 Catalytic Subunit, CAPN2 ELISA Kit

Rat Calpain-2 Catalytic Subunit, CAPN2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Calpain-2 Catalytic Subunit

Code

E1325Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.

UniProt accession

MASS(Da)

79,919

GeneID

29154

Synonyms

Calcium-activated neutral proteinase 2;Calpain M-type;Calpain-2 catalytic subunit;Calpain-2 large subunit;CANP 2;CAPN 2;CAPN2;M-calpain;Millimolar-calpain;

Gene names

CAPN2

Research area

Signal Transduction

Target protein

CAPN2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1325Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.336 2.206 2.167
2.076
8ng/ml 1.933 1.923 1.885
1.914
4ng/ml 1.232 1.267 1.229
1.302
2ng/ml 0.608 0.603 0.565
0.598
1ng/ml 0.371 0.369 0.331
0.368
0ng/ml 0.041 0.038 0
0.035

Precision

We measured random samples of E1325Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.3 0.14 3.4
Intra-Assay 2 18 1.8 0.08 4.4
Intra-Assay 3 18 2.4 0.11 4.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1325Ra

    $320.00

  • 96T

    E1325Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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