Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Rat Heat Shock Protein 72, HSP72 ELISA Kit

Rat Heat Shock Protein 72, HSP72 ELISA Kit

Rat Heat Shock Protein 72, HSP72 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Heat Shock Protein 72

Code

E1348Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.26ng/ml

Detection range

0.5-100ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells.

UniProt accession

MASS(Da)

69,642

GeneID

60460

Synonyms

Heat shock protein 70.2;Heat shock-related 70 kDa protein 2;HSPA 2;HSPA2;HST;Testis-specific heat shock protein-related

Gene names

HSPA2

Research area

Others

Target protein

HSPA2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1348Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
64ng/ml 2.269 2.306 2.252
2.343
32ng/ml 1.358 1.413 1.359
1.468
16ng/ml 0.787 0.771 0.718
0.756
8ng/ml 0.428 0.486 0.432
0.544
4ng/ml 0.263 0.249 0.195
0.235
0ng/ml 0.048 0.054 0
0.059

Precision

We measured random samples of E1348Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 34.8 1.27 3.6
Intra-Assay 2 18 24.9 1.36 5.5
Intra-Assay 3 18 6.9 0.34 5

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E1348Ra

    $320.00

  • 96T

    E1348Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up