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Home > Product > ELISA Kit > Rat Apolipoprotein B, APO-B ELISA Kit

Rat Apolipoprotein B, APO-B ELISA Kit

Rat Apolipoprotein B, APO-B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.02mmol/L

Detection range: 0.05 -3mmol/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.02mmol/L

Detection range: 0.05 -3mmol/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Apolipoprotein B

Code

E1356Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.02mmol/L

Detection range

0.05 -3mmol/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

UniProt accession

MASS(Da)

536,024

GeneID

54225

Synonyms

Apo B-100;Apo B-48;APOB;Apolipoprotein B-100;Apolipoprotein B-48

Gene names

APOB

Research area

Cardiovascular; Signal Transduction

Target protein

APOB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1356Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1.6mmol/L 2.42 2.357 2.299
2.295
0.8mmol/L 1.346 1.377 1.319
1.409
0.4mmol/L 0.813 0.794 0.736
0.776
0.2mmol/L 0.43 0.431 0.373
0.431
0.1mmol/L 0.261 0.272 0.213
0.282
0mmol/L 0.07 0.058 0
0.046

Precision

We measured random samples of E1356Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 0.6 0.04 5.6
Intra-Assay 2 18 1 0.03 3.3
Intra-Assay 3 18 0.3 0.02 6.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E1356Ra

    $320.00

  • 96T

    E1356Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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