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Home > Product > ELISA Kit > Rat Nephrin, NPHN ELISA Kit

Rat Nephrin, NPHN ELISA Kit

Rat Nephrin, NPHN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.109ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.109ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Nephrin

Code

E1366Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.109ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.

UniProt accession

MASS(Da)

136,281

GeneID

64563

Synonyms

Nephrin;NPHS 1;NPHS1;Renal glomerulus-specific cell adhesion receptor

Gene names

NPHS1

Research area

Signal Transduction

Target protein

NPHS1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1366Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.274 2.272 2.228
2.271
20ng/ml 1.147 1.265 1.222
1.383
10ng/ml 0.636 0.637 0.593
0.637
5ng/ml 0.489 0.479 0.435
0.469
2.5ng/ml 0.18 0.194 0.15
0.208
0ng/ml 0.045 0.044 0
0.042

Precision

We measured random samples of E1366Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.5 0.45 5.9
Intra-Assay 2 18 17.9 1.02 5.7
Intra-Assay 3 18 28.2 0.82 2.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E1366Ra

    $320.00

  • 96T

    E1366Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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