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Home > Product > ELISA Kit > Bovine Anti-Thrombin 3, AT-3 ELISA Kit

Bovine Anti-Thrombin 3, AT-3 ELISA Kit

Bovine Anti-Thrombin 3, AT-3 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 10.04ng/ml

Detection range: 20-6000ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 10.04ng/ml

Detection range: 20-6000ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Anti-Thrombin 3

Code

E0195Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

10.04ng/ml

Detection range

20-6000ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin (By similarity).

UniProt accession

MASS(Da)

52,347

GeneID

540261

Synonyms

Antithrombin-III;AT3;ATIII;Serpin C1;SERPINC 1;SERPINC1;THPH7

Gene names

SERPINC1

Research area

Cardiovascular

Target protein

SERPINC1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0195Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/ml 1.936 2.045 2.005
2.154
1600ng/ml 1.014 1.037 0.997
1.06
800ng/ml 0.387 0.468 0.429
0.549
400ng/ml 0.287 0.243 0.203
0.199
200ng/ml 0.141 0.106 0.067
0.072
0ng/ml 0.037 0.04 0
0.043

Precision

We measured random samples of E0195Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2630.8 48.6 1.8
Intra-Assay 2 18 752.4 34.78 4.6
Intra-Assay 3 18 1221.1 41.38 3.4

Protocols

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  • Size

    Code

    Price

  • 96T

    E0195Bo

    $458.00

  • 48T

    E0195Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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