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Home > Product > ELISA Kit > Human Bactericidal Permeability-increasing Protein, BPI ELISA Kit

Human Bactericidal Permeability-increasing Protein, BPI ELISA Kit

Human Bactericidal Permeability-increasing Protein, BPI ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.28ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.28ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Bactericidal Permeability-Increasing Protein

Code

E0323Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.28ng/ml

Detection range

0.5-150ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.

UniProt accession

MASS(Da)

53,900

GeneID

671

Synonyms

Bactericidal permeability-increasing protein;BPI;CAP 57

Gene names

BPI

Research area

Immunology

Target protein

BPI

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0323Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
80ng/ml 2.101 2.206 2.148
2.312
40ng/ml 1.354 1.374 1.315
1.393
20ng/ml 0.707 0.752 0.693
0.797
10ng/ml 0.522 0.494 0.436
0.466
5ng/ml 0.306 0.297 0.239
0.288
0ng/ml 0.05 0.058 0
0.067

Precision

We measured random samples of E0323Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 49.8 2.07 4.2
Intra-Assay 2 18 13.7 0.97 7.1
Intra-Assay 3 18 22.8 1.18 5.2

Protocols

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  • Size

    Code

    Price

  • 96T

    E0323Hu

    $458.00

  • 48T

    E0323Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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