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Home > Product > ELISA Kit > Rat Sortilin, SORT1 ELISA Kit

Rat Sortilin, SORT1 ELISA Kit

Rat Sortilin, SORT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.94ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.94ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Sortilin

Code

E1429Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.94ng/L

Detection range

2-600ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Lysosomal proteins bind specifically to the receptor in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin (By similarity). May also mediate transport from the endoplasmic reticulum to the Golgi (PubMed: 12771154).

UniProt accession

MASS(Da)

91,169

GeneID

83576

Synonyms

Glycoprotein 110;Gp110;Neurotensin receptor 3;NTR3;SORT 1;SORT1;Sortilin

Gene names

SORT1

Research area

Others

Target protein

SORT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1429Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
320ng/L 2.16 2.236 2.2
2.313
160ng/L 1.388 1.387 1.351
1.386
80ng/L 0.958 0.939 0.902
0.919
40ng/L 0.437 0.427 0.39
0.416
20ng/L 0.305 0.301 0.264
0.297
0ng/L 0.038 0.037 0
0.035

Precision

We measured random samples of E1429Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 88.7 5.54 6.2
Intra-Assay 2 18 193.4 8.02 4.1
Intra-Assay 3 18 43.9 2.86 6.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1429Ra

    $320.00

  • 96T

    E1429Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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