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Home > Product > ELISA Kit > Human Complement Factor H, CFH ELISA Kit

Human Complement Factor H, CFH ELISA Kit

Human Complement Factor H, CFH ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 4.88ng/ml

Detection range: 10-3000ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 4.88ng/ml

Detection range: 10-3000ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Complement Factor H

Code

E0324Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

4.88ng/ml

Detection range

10-3000ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces (PubMed: 21285368, PubMed: 25402769). Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop (PubMed: 19503104). As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b (PubMed: 18252712, PubMed: 28671664). In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed (PubMed: 9558116, PubMed: 20008295).

UniProt accession

MASS(Da)

139,096

GeneID

3075

Synonyms

CFH;Complement factor H;H factor 1

Gene names

CFH

Research area

Immunology

Target protein

CFH

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0324Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/ml 2.353 2.36 2.333
2.367
800ng/ml 1.205 1.256 1.229
1.307
400ng/ml 0.72 0.718 0.691
0.716
200ng/ml 0.455 0.448 0.421
0.441
100ng/ml 0.171 0.172 0.145
0.173
0ng/ml 0.028 0.027 0
0.026

Precision

We measured random samples of E0324Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 717.3 40.3 5.6
Intra-Assay 2 18 1115.7 40.83 3.7
Intra-Assay 3 18 255.3 14.54 5.7

Protocols

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  • Size

    Code

    Price

  • 96T

    E0324Hu

    $458.00

  • 48T

    E0324Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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