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Home > Product > ELISA Kit > Rat Vascuoar Endothelial Cell Growth Factor Receptor 3, VEGFR-3 ELISA Kit

Rat Vascuoar Endothelial Cell Growth Factor Receptor 3, VEGFR-3 ELISA Kit

Rat Vascuoar Endothelial Cell Growth Factor Receptor 3, VEGFR-3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.41ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 1.41ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Vascuoar Endothelial Cell Growth Factor Receptor 3

Code

E1461Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.41ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473' (By similarity).

UniProt accession

MASS(Da)

153,317

GeneID

114110

Synonyms

FLT 4;FLT4;FLT-4;Fms-like tyrosine kinase 4;Tyrosine-protein kinase receptor FLT4;Vascular endothelial growth factor receptor 3;VEGFR-3

Gene names

FLT4

Research area

Signal Transduction

Target protein

FLT4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1461Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 1.973 2.006 1.967
2.038
240ng/L 1.407 1.37 1.331
1.332
120ng/L 0.727 0.752 0.713
0.777
60ng/L 0.326 0.366 0.327
0.407
30ng/L 0.162 0.155 0.116
0.148
0ng/L 0.024 0.039 0
0.054

Precision

We measured random samples of E1461Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 172.8 9.57 5.5
Intra-Assay 2 18 220.9 8.21 3.7
Intra-Assay 3 18 83.1 4.54 5.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1461Ra

    $320.00

  • 96T

    E1461Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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