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Home > Product > ELISA Kit > Rat Peroxiredoxin 1, PRDX1 ELISA Kit

Rat Peroxiredoxin 1, PRDX1 ELISA Kit

Rat Peroxiredoxin 1, PRDX1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.092ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.092ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Peroxiredoxin 1

Code

E1591Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.092ng/ml

Detection range

0.2-70ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (By similarity). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).

UniProt accession

MASS(Da)

22,109

GeneID

117254

Synonyms

HBP23;Heme-binding 23 kDa protein;Peroxiredoxin-1;PRDX 1;PRDX1;Thioredoxin peroxidase 2;Thioredoxin-dependent peroxide reductase 2;Thioredoxin-dependent peroxiredoxin 1

Gene names

PRDX1

Research area

Cell Biology; Cancer; Metabolism

Target protein

PRDX1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1591Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.314 2.367 2.305
2.419
20ng/ml 1.266 1.262 1.2
1.258
10ng/ml 0.764 0.748 0.687
0.733
5ng/ml 0.428 0.454 0.393
0.481
2.5ng/ml 0.291 0.309 0.247
0.326
0ng/ml 0.076 0.062 0
0.047

Precision

We measured random samples of E1591Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 26.3 0.71 2.7
Intra-Assay 2 18 18.8 1.09 5.8
Intra-Assay 3 18 5.8 0.37 6.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E1591Ra

    $320.00

  • 96T

    E1591Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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