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Home > Product > ELISA Kit > Rat Neurofilament Light Polypeptide, NEFL ELISA Kit

Rat Neurofilament Light Polypeptide, NEFL ELISA Kit

Rat Neurofilament Light Polypeptide, NEFL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.67ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.67ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Neurofilament Light Polypeptide

Code

E1747Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.67ng/L

Detection range

1-400ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

UniProt accession

MASS(Da)

61,335

GeneID

83613

Synonyms

68 kDa neurofilament protein;NEFL;Neurofilament light polypeptide;Neurofilament triplet L protein;NF-L

Gene names

NEFL

Research area

Neuroscience

Target protein

NEFL

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1747Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/L 2.087 2.111 2.071
2.136
120ng/L 1.102 1.13 1.089
1.159
60ng/L 0.516 0.484 0.443
0.453
30ng/L 0.192 0.222 0.181
0.251
15ng/L 0.114 0.138 0.097
0.162
0ng/L 0.056 0.041 0
0.025

Precision

We measured random samples of E1747Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 127.4 5.59 4.4
Intra-Assay 2 18 172.3 4.87 2.8
Intra-Assay 3 18 58 1.98 3.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E1747Ra

    $320.00

  • 96T

    E1747Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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