Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Rat Tissue-type Plasminogen Activator, TPA ELISA Kit

Rat Tissue-type Plasminogen Activator, TPA ELISA Kit

Rat Tissue-type Plasminogen Activator, TPA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.04ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.04ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Tissue-Type Plasminogen Activator

Code

E1758Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.04ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (PubMed: 1515147).

UniProt accession

MASS(Da)

62,903

GeneID

25692

Synonyms

PLAT;Tissue-type plasminogen activator;Tissue-type plasminogen activator chain A;Tissue-type plasminogen activator chain B;tPA;t-PA;t-plasminogen activator

Gene names

PLAT

Research area

Cardiovascular

Target protein

PLAT

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1758Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.399 2.533 2.476
2.667
12ng/ml 1.576 1.493 1.436
1.411
6ng/ml 0.912 0.876 0.818
0.839
3ng/ml 0.496 0.495 0.437
0.494
1.5ng/ml 0.207 0.218 0.16
0.229
0ng/ml 0.054 0.057 0
0.06

Precision

We measured random samples of E1758Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.5 0.41 5.5
Intra-Assay 2 18 14 0.49 3.5
Intra-Assay 3 18 2.2 0.13 5.9

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E1758Ra

    $320.00

  • 96T

    E1758Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up