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Home > Product > ELISA Kit > Human Toll-like Receptor 4, TLR4 ELISA Kit

Human Toll-like Receptor 4, TLR4 ELISA Kit

Human Toll-like Receptor 4, TLR4 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.027ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.027ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Toll-Like Receptor 4

Code

E0346Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.027ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed: 27022195). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed: 9237759, PubMed: 10835634, PubMed: 27022195,PubMed: 21393102). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species-specific (PubMed: 20711192). Both M.tuberculosis HSP70 (dnaK) and HSP65 (groEL-2) act via this protein to stimulate NF-kappa-B expression (PubMed: 15809303). In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed: 23880187). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via TLR2, but also partially via this receptor (PubMed: 16622205, PubMed: 10835634, PubMed: 15809303, PubMed: 17478729, PubMed: 20037584, PubMed: 20711192, PubMed: 23880187, PubMed: 27022195, PubMed: 9237759). Activated by the signaling pathway regulator NMI which acts as damage-associated molecular patterns (DAMPs) in response to cell injury or pathogen invasion, therefore promoting nuclear factor NF-kappa-B activation (PubMed: 29038465).

UniProt accession

MASS(Da)

95,680

GeneID

7099

Synonyms

CD antigen CD284;hToll;TLR 4;TLR4;Toll-like receptor 4

Gene names

TLR4

Research area

Immunology; Microbiology; Cardiovascular

Target protein

TLR4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0346Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.28 2.138 2.105
1.996
4ng/ml 1.556 1.445 1.412
1.334
2ng/ml 0.551 0.606 0.573
0.661
1ng/ml 0.389 0.356 0.323
0.323
0.5ng/ml 0.147 0.136 0.103
0.124
0ng/ml 0.043 0.033 0
0.023

Precision

We measured random samples of E0346Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.8 0.11 3
Intra-Assay 2 18 3.4 0.16 4.6
Intra-Assay 3 18 1.9 0.07 3.5

Protocols

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  • Size

    Code

    Price

  • 96T

    E0346Hu

    $458.00

  • 48T

    E0346Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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