Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Rat Hypoxanthine-Guanine Phosphoribosyltransferase, HPRT1 ELISA Kit

Rat Hypoxanthine-Guanine Phosphoribosyltransferase, HPRT1 ELISA Kit

Rat Hypoxanthine-Guanine Phosphoribosyltransferase, HPRT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.081ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.081ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Hypoxanthine-Guanine Phosphoribosyltransferase

Code

E1802Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.081ng/ml

Detection range

0.2-70ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).

UniProt accession

MASS(Da)

24,477

GeneID

10368998324465

Synonyms

HGPRT;HGPRTase;HPRT 1;HPRT1;Hypoxanthine-guanine phosphoribosyltransferase

Gene names

HPRT1

Research area

Epigenetics and Nuclear Signaling

Target protein

HPRT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1802Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
36ng/ml 2.055 2.16 2.115
2.264
18ng/ml 1.252 1.214 1.17
1.176
9ng/ml 0.808 0.733 0.688
0.658
4.5ng/ml 0.433 0.467 0.423
0.502
2.25ng/ml 0.189 0.196 0.152
0.203
0ng/ml 0.057 0.044 0
0.032

Precision

We measured random samples of E1802Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.7 0.38 5.7
Intra-Assay 2 18 19.4 0.69 3.6
Intra-Assay 3 18 17.1 1.12 6.6

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E1802Ra

    $320.00

  • 96T

    E1802Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up