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Home > Product > ELISA Kit > Bovine Interleukin 1Β, IL-1B ELISA Kit

Bovine Interleukin 1Β, IL-1B ELISA Kit

Bovine Interleukin 1Β, IL-1B ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.25ng/L

Detection range: 0.5-200ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 0.25ng/L

Detection range: 0.5-200ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Interleukin 1beta

Code

E0197Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.25ng/L

Detection range

0.5-200ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.

UniProt accession

MASS(Da)

30,760

GeneID

281251

Synonyms

IL 1B;IL 1beta;IL-1 beta;IL1B;Interleukin-1 beta

Gene names

IL1B

Research area

Immunology; Microbiology; Cardiovascular; Metabolism

Target protein

IL1B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0197Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/L 2.276 2.315 2.276
2.355
60ng/L 1.384 1.286 1.247
1.187
30ng/L 0.61 0.69 0.651
0.771
15ng/L 0.301 0.317 0.278
0.333
7.5ng/L 0.206 0.196 0.157
0.186
0ng/L 0.024 0.039 0
0.054

Precision

We measured random samples of E0197Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 84.9 2.41 2.8
Intra-Assay 2 18 31.5 1.97 6.2
Intra-Assay 3 18 51.3 2.79 5.4

Protocols

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  • Size

    Code

    Price

  • 96T

    E0197Bo

    $458.00

  • 48T

    E0197Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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